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建立了采用超高效色谱(UPLC)-荧光检测器法测定柴油中10种常见的多环芳烃含量的方法。这10种常见的多环芳烃为萘、苊、二氢苊、芴、菲、蒽、荧蒽、芘、苯并[a]蒽、艹屈、苯并[b]荧蒽、苯并[k]荧蒽、苯并[a]芘和苯并[g,h,i]芘。样品经过固相萃取后用甲醇溶解,采用Acquity UPLC BEH Phenyl C18柱(100 mm×2.1 mm,1.7μm)分离,乙腈-水为流动相进行梯度洗脱,采用荧光检测器检测,并用外标法进行定量分析。结果表明,在一定质量浓度范围内,峰面积与质量浓度的线性关系良好,用标准加入法进行回收率实验,对方法的准确度进行考察,相对标准偏差为0.56%~8.76%,加标回收率为100.04%~115.04%。与其他检测柴油中多环芳烃方法比较,该方法简单,分离效果好,快速及准确。
A method for the determination of 10 common polycyclic aromatic hydrocarbons (PAHs) in diesel fuel by UPLC-fluorescence detector was established. The 10 common polycyclic aromatic hydrocarbons are naphthalene, acenaphthene, dihydroanthracene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo [a] anthracene, ] Fluoranthene, benzo [a] pyrene and benzo [g, h, i] pyrene. The sample was dissolved in methanol after solid-phase extraction and separated on an Acquity UPLC BEH Phenyl C18 column (100 mm × 2.1 mm, 1.7 μm). The sample was eluted with acetonitrile-water as mobile phase and detected by fluorescence detector. Quantitative analysis. The results showed that there was a good linear relationship between peak area and mass concentration in a certain range of mass concentration. The recovery rate was tested by standard addition method, and the accuracy of the method was investigated. The relative standard deviations were 0.56% ~ 8.76% The rates ranged from 100.04% to 115.04%. Compared with other methods for detecting PAHs in diesel oil, the method is simple, the separation effect is good, fast and accurate.