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以地高辛为标记物 ,利用PCR方法制备 16SrRNA基因探针与ctxA基因探针 ,经Southern杂交 ,对霍乱弧菌进行分型。对广东地区分离自病人的 6 0株埃尔托型 (EVC)、10株O139群和 2株标准株进行研究。经 16SrRNA基因在探针共分为 4个类型 (A、B、C和D) ,EVC和O139群均以D型为优势克隆。经ctxA基因探针Southern杂交 ,共分为A和B型 ,2株分离自 1998年的EVC为A型 ,其余EVC和O139群均为B型。与国内外资料比较 ,广东地区霍乱弧菌的优势克隆与国内其它地区及东南亚地区的基本一致。
Using digoxigenin as a marker, 16S rRNA gene probe and ctxA gene probe were prepared by PCR method. Vibrio cholerae were typed by Southern hybridization. Sixty elto-type (EVC), ten O139 and two standard strains isolated from patients in Guangdong were studied. The 16SrRNA gene was divided into four types (A, B, C and D) in the probe, and the EVC and O139 clones were predominantly D type. The ctxA gene probe Southern hybridization, divided into A and B type, two from EVC in 1998 for the A type, the remaining EVC and O139 group are B type. Compared with the data at home and abroad, the dominant clone of Vibrio cholerae in Guangdong is basically consistent with other regions in China and Southeast Asia.