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目的探讨三氧化二砷(As_2O_3)磁性四氧化三铁(Fe_3O_4)白蛋白微球对人卵巢癌细胞耐药性的作用及其可能机制。方法体外培养人卵巢癌顺铂耐药细胞株SKOV3/CDDP,随机分为阴性对照组(A组)、交变磁场(AMF)组(B组)、Fe_3O_4白蛋白微球组(C组)、Fe_3O_4白蛋白微球联合AMF组(D组)、As_2O_3白蛋白微球组(E组)、As_2O_3磁性Fe_3O_4白蛋白微球联合AMF组(F组)和顺铂溶液组(G组)。采用MTT法和流式细胞术分别检测细胞生长和凋亡情况,RT-PCR法检测凋亡相关基因Bcl-2、Bax和Caspase-3mRNA表达,Western blot法检测多重耐药蛋白1(MDR1)、多重耐药相关蛋白2(MRP2)的蛋白表达。结果A、B、C组间细胞生长和凋亡情况无统计学差异(P>0.05)。与A组和G组相比,D、E、F组细胞生长抑制率和凋亡率增加,Bax和Caspase-3mRNA表达升高,Bcl-2mRNA以及MDR1、MRP2蛋白表达降低(P<0.05),F组各指标变化更为明显(P<0.05)。结论 As_2O_3磁性Fe_3O_4白蛋白微球联合AMF能有效逆转SKOV3/CDDP细胞耐药性;其作用机制可能与下调Bcl-2、MDR1、MRP2以及上调Bax、Caspase-3的表达有关。
Aim To investigate the effect of magnetic arsenic trioxide (Fe_3O_4) albumin microspheres on the drug resistance of human ovarian cancer cells and its possible mechanism. Methods Human ovarian cancer cell line SKOV3 / CDDP was cultured in vitro and divided into three groups randomly: negative control group (A group), alternating magnetic field (AMF group), Fe3O4 albumin microsphere group (C group) Fe 3 O 4 albumin microspheres combined with AMF group (group D), As 2 O 3 albumin microsphere group (group E), As 2 O 3 magnetic Fe 3 O 4 albumin microsphere combined with AMF group (F group) and cisplatin solution group (G group). The cell growth and apoptosis were detected by MTT assay and flow cytometry respectively. The expressions of Bcl-2, Bax and Caspase-3 mRNA were detected by RT-PCR and Western blotting respectively. The expressions of MDR1, Multidrug resistance-associated protein 2 (MRP2) protein expression. Results There was no significant difference in cell growth and apoptosis between A, B and C groups (P> 0.05). Compared with group A and group G, the growth inhibition rates and apoptosis rates of groups D, E and F increased, the expressions of Bax and Caspase-3 mRNA increased, the expressions of Bcl-2 mRNA, MDR1 and MRP2 decreased (P <0.05) The indexes of group F changed more obviously (P <0.05). Conclusions As_2O_3 magnetic Fe_3O_4 albumin microspheres combined with AMF can effectively reverse the drug resistance of SKOV3 / CDDP cells. The mechanism may be related to the down-regulation of Bcl-2, MDR1, MRP2 and the up-regulation of Bax and Caspase-3 expression.