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目的：了解次声引起应激反应的中枢机制．方法：采用反转录ＰＣＲ方法，研究了８Ｈｚ，１２０ｄＢ次声作用后不同时间引起大鼠下丘脑ｃ－ｆｏｓｍＲＮＡ及促肾上腺皮质激素释放因子（ＣＲＦ）ｍＲＮＡ的表达情况．结果：８Ｈｚ，１２０ｄＢ次声作用２ｈ，于作用后０．５ｈｃ－ｆｏｓ与ＣＲＦｍＲＮＡ均开始明显表达，随时间延长，表达渐增强，ｃ－ｆｏｓ于２ｈ达高峰，至４ｈ基本消失；ＣＲＦ于３ｈ达高峰，至５ｈ基本消失．结论：次声作用后ｃ－ｆｏｓ与ＣＲＦｍＲＮＡ的表达可能是次声引起应激反应的中枢分子机制．
Objective: To understand the central mechanism of infrasound-induced stress response. Methods: Reverse transcription PCR was used to study the expression of c-fos mRNA and corticotropin-releasing factor (CRF) mRNA in rat hypothalamus at different time after 8Hz and 120dB infrasound. Results: After acupuncture at 120 Hz and 8 Hz for 2 hours, the expressions of both 0.5 hc-fos and CRF mRNA began to be significantly increased. The expression of c-fos reached its peak at 2 h and disappeared at 4 h. Peak, to 5h basically disappeared. CONCLUSION: The expression of c-fos and CRF mRNA after infrasound may be the central molecular mechanism of infrasound-induced stress response.