论文部分内容阅读
用改良Iscove甲基纤维素法体外培养小鼠骨髓红系祖细胞,对形成的红系集落细胞分化进行形态学观察。结果发现:(1)在低浓度红细胞生成素培养条件下,处于较晚发育期的红系祖细胞(CFU-E)可形成数十个细胞组成的集落,但各集落间的细胞数量及发育阶段的同步性有较大差异;(2)在高浓度红细胞生成素培养条件下,处于较早发育期的红系祖细胞(BFU-E)可形成数千个甚至上万个细胞组成的大集落,且集落内细胞的发育阶段具较高的同步性;(3)在CFu-E和BFu-E培养集落中均可观察到自然出现的核固缩及排核现象。以上发现为哺乳类红细胞自然排核学说及红系终末细胞中存在不利于细胞核增殖生长的“胞质因子”工作假说提供了有利的佐证。
Mice bone marrow erythroid progenitor cells were cultured in vitro with the modified Iscove methylcellulose method. Morphological observation of the erythroid colony formed. The results showed that: (1) Under the condition of low concentration of erythropoietin, the erythroid progenitor cells (CFU-E) at the late development stage could form colonies of dozens of cells, but the number and development of cells (2) In the condition of high concentration of erythropoietin, the erythroid progenitor cells (BFU-E) at an early stage of development can form large or even tens of thousands of cells (3) Naturally occurring nuclear pyknosis and nuclear extinction were observed in CFu-E and BFu-E cultured colonies. The above findings provide favorable evidence for the natural nuclear exclusion theory of mammalian erythrocytes and the working hypothesis of “cytokines” that are unfavorable to the proliferation and proliferation of the nucleus in the erythroid terminal cells.