论文部分内容阅读
【目的】探讨枸杞多糖(lycium barbarum polysaccharides,LBP)对大鼠脑胶质瘤细胞株 C6增殖及凋亡的影响。【方法】在倒置显微镜下观察脑胶质瘤细胞凋亡的形态学变化；MTT 法检测 LBP 对 C6细胞增殖抑制率的变化；用流式细胞仪检测细胞凋亡率和细胞周期的变化；免疫细胞化学法测定 C6细胞中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达变化。【结果】经 LBP 诱导后的 C6出现细胞形态不规则,变圆并脱落的现象；LBP 能抑制 C6细胞的增殖能力,且呈剂量依赖性；LBP 作用后 C6的细胞周期更多地被阻止于 G2/M 期；LBP 作用后的脑胶质瘤 C6细胞中 PCNA 表达水平下降。【结论】LBP 可抑制大鼠脑胶质瘤 C6细胞增殖并使其凋亡,其作用机制可能是 LBP 可下调大鼠脑胶质瘤 C6细胞 PCNA 的表达水平。“,”[Objective]To explore the proliferation inhibition and apoptosis mechanism of Lycium barba-rum polysaccharides (LBP)on C6 glioma cells in rats.[Methods]The morphological changes of apoptosis was observed by inverted microscope.And the proliferation inhibitory rate was detected by methyl thiazolyl tetrazo-lium (MTT).Cell apoptosis rate and cell cycle were detected by flow cytometry.The expression level of prolif-erating cell nuclear antigen (PCNA)was detected by immunocytochemistry.[Results]The cells became irreg-ular-shaped,round and sloughed off by the induction of LBP.And LBP could inhibit the cell proliferation abili-ty in a dose dependent fashion.Cell cycle was arrested in G2/M phase.And there was a down-regulation of PC-NA.[Conclusion]LBP can inhibit the cell proliferation and apoptosis.And the mechanism may be through a LBP-mediated down-regulation of PCNA in rat C6 cells.