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目的:探讨Ｌ－精氨酸(Ｌ－Arg)对大鼠血管球囊损伤后病理学变化,细胞周期依赖性激酶2(CDK２)、细胞周期蛋白E(CyclinE)、增殖细胞核抗原(PCNA)的表达和血小板颗粒膜蛋白(GMP－140)、D－二聚体(D－dimer)含量的影响。方法:63只大鼠,随机分为对照组(n＝7),手术组(n＝28)和Ｌ－Arg组(n＝28),监测以上各项指标。结果:①对照组动脉腔内无新生内膜;手术组动脉腔内有明显新生内膜;Ｌ－Arg组新生内膜明显减轻。新生内膜面积及内膜/中膜面积,Ｌ－Arg组较手术组分别降低59．1%及62．9%(均为P＜0.01)。②对照组COK2、CyclinE和PCNA表达呈阴性,手术组均呈强阳性,Ｌ－Arg组表达减少。③手术组血浆NO水平低于对照组及Ｌ－Arg组(P均＜0．01),而血浆GMP-140和D-dimer显著高于对照组(P＜0．001)和Ｌ－Arg组(P＜0．01)。④血浆GMP-140与D－dimer含量与新生内膜面积及内膜/中膜面积比均呈显著正相关,r分别为0.89(P＜0.01)、0.86(P＜0．05)和0.79、0．84(P＜0．05)。结论:Ｌ－Arg能有效抑制血管内膜损伤后的内膜增生,其机制可能与逆转CDK2、CyclinE的过度表达和抑制血小板活化、血栓形成有关。
Objective: To investigate the effects of L-arginine on the pathological changes, the expression of CDK2, CyclinE, PCNA in rats after vascular balloon injury Expression and platelet granule membrane protein (GMP-140), D-dimer (D-dimer) content. Methods: Sixty-three rats were randomly divided into control group (n = 7), operation group (n = 28) and L-Arg group (n = 28) Results: ① There was no neointima in the arterial cavity of the control group. The neointima was found in the arterial cavity of the operation group. The neointima of L-Arg group was significantly reduced. Neointimal area and intima / media area, L-Arg group decreased by 59.1% and 62.9% respectively compared with the operation group (all P <0.01). ② The expression of COK2, CyclinE and PCNA in the control group was negative, and the expression in the L-Arg group was significantly decreased. ③ The levels of plasma NO in the operation group were lower than those in the control group and the L-Arg group (P <0.01), while the levels of GMP-140 and D-dimer in the plasma were significantly higher than those in the control group (P <0.001) (P <0.01). Plasma GMP-140 and D-dimer levels were positively correlated with neointimal area and intima / media area ratio (r = 0.89, P <0.01) 0.84 (P <0.05). Conclusion: L-Arg can effectively inhibit intimal hyperplasia after vascular intimal injury. The mechanism may be related to reversing the over-expression of CDK2 and CyclinE and inhibiting platelet activation and thrombosis.