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目的 定性检测胃癌患者外周血CEA(癌胚抗原 )mRNA表达 ,对预测胃癌微转移的意义。方法 应用RT -PCR(逆转录 -聚合酶链反应 )、ELISA(酶联免疫吸附试验 )同时检测 40例胃癌、14例胃炎患者及 2 0例健康成人对照外周血CEAmRNA和CEA蛋白。结果 40例胃癌患者CEAmRNA阳性率为 6 0 % (2 4/ 40 ) ,而健康者对照均为阴性。外周血CEAmRNA的表达与胃癌远处转移密切相关 ,且随着病期进展而增加。结论 CEAmRNA检测是预测胃癌微转移的有效指标 ,血中CEAmRNA的阳性提示肿瘤的早期转移
Objective To qualitatively detect the peripheral blood CEA (carcinoembryonic antigen) mRNA expression in gastric cancer patients and to predict the significance of micrometastasis in gastric cancer. Methods RT-PCR (reverse transcription polymerase chain reaction) and ELISA (enzyme-linked immunosorbent assay) were used to simultaneously detect CEAmRNA and CEA protein in 40 cases of gastric cancer, 14 cases of gastritis and 20 healthy adults. Results The positive rate of CEAmRNA in 40 patients with gastric cancer was 60% (24/40), while the controls in healthy individuals were negative. The expression of CEAmRNA in peripheral blood is closely related to the distant metastasis of gastric cancer, and it increases with the progression of the disease. Conclusion The detection of CEAmRNA is an effective index to predict the micrometastasis of gastric cancer. The positive expression of CEAmRNA in blood suggests early metastasis of tumor.