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目的:应用高效液相色谱法测定茸血补心丸中大黄素的含量。方法:采用Agilent C18柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸溶液(42∶58)为流动相,检测波长为254 nm。结果:大黄素平均回收率为99.4%,RSD=1.4%(n=6),线性范围为0.05~0.6μg(r=0.999 98)。结论:该方法重复性好,结果准确,可用于茸血补心丸的质量控制。
Objective: To determine the content of emodin in Velvet Shuangxin Capsule by high performance liquid chromatography. Methods: Agilent C18 column (4.6 mm×250 mm, 5 μm) was used, and the mobile phase was acetonitrile-0.1% phosphoric acid solution (42:58). The detection wavelength was 254 nm. Results: The average recovery rate of emodin was 99.4%, RSD was 1.4% (n=6), and the linear range was 0.05-0.6 μg (r=0.999 98). Conclusion: This method has good reproducibility and accurate results and can be used for the quality control of Rongshenbuxin Pill.