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目的:研究淫羊藿苷(icariin,ICA)对MCF-7细胞增殖/凋亡作用的影响及其作用机制。方法:采用MTT比色分析法及Annexin V/PI标记流式细胞仪检测ICA对MCF-7细胞的增殖与凋亡作用的影响,采用Western blot检测蛋白表达,研究ICA是否通过ERK/MAPK信号转导通路起作用。结果:与对照组比较,MCF-7细胞培养24 h后,ICA各组OD值均降低,差异有统计学意义(P<0.05);培养48 h后,各组差异均有统计学意义(P<0.05);培养72 h后,各组差异均有统计学意义(P<0.05)。与对照组比较,培养24 h后,10 m M ES单独作用后对MCF-7细胞无明显的增殖作用,ES加ICA组有明显的抑制作用。20 m M ES诱导结果与10 m M的结果一致。不同浓度的ICA作用48 h后,总ERK1/2和MEK蛋白表达均无明显变化,MEK磷酸化水平同样无明显变化,ERK1/2磷酸化水平增高。结论:淫羊藿苷对雌激素阳性人乳腺癌细胞有明显的抑制增殖与诱导凋亡的作用,同时可拮抗雌激素对乳腺癌细胞的增殖作用,但是其作用机制尚不清楚。
Objective: To investigate the effect of icariin (ICA) on the proliferation and apoptosis of MCF-7 cells and its mechanism. Methods: The effects of ICA on the proliferation and apoptosis of MCF-7 cells were detected by MTT colorimetric assay and Annexin V / PI flow cytometry. Western blot was used to detect the expression of ICA by ERK / MAPK signal transduction Conduction path to work. Results: Compared with the control group, the OD value of each group of ICA decreased after 24 h incubation in MCF-7 cells, the difference was statistically significant (P <0.05); after 48 h, the differences were statistically significant (P <0.05). After culturing for 72 h, there was significant difference among all groups (P <0.05). Compared with the control group, MCF-7 cells had no significant proliferative effects after 10 m M ES alone, while ES plus ICA group had significant inhibitory effects after cultured for 24 h. The 20 m M ES induction results were consistent with 10 m M results. At different concentrations of ICA for 48 h, the expression of total ERK1 / 2 and MEK protein had no obvious change, the phosphorylation of MEK also had no obvious change, and the phosphorylation of ERK1 / 2 increased. CONCLUSION: Icariin can significantly inhibit the proliferation and induce apoptosis of estrogen-positive human breast cancer cells and antagonize the proliferation of breast cancer cells induced by estrogen. However, its mechanism of action is not clear.