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以转单价Bt基因抗虫棉品系中心Bt和常规棉苏棉 12号为对照 ,研究了转Bt+CpTI双抗 1抗虫棉对棉铃虫抗性的时空表达特征。在棉花生长发育进程中 ,用 4～ 2 1叶位主茎叶饲喂棉铃虫初孵幼虫 ,5天后观察 :双抗 1和中心Bt的 4～ 2 1叶上存活棉铃虫中均无 3龄幼虫 ,苏棉 12号中 3龄幼虫占存活幼虫的 4 0 %以上 ;双抗 1和中心Bt棉铃虫的死亡率相近且随叶位升高而下降。分期播种抗虫性测验与各叶位抗虫性测定相似 ,即双抗 1和中心Bt各播种期的存活棉铃虫中没有 3龄虫 ,苏棉 12号各播种期都有 3龄虫出现 ,双抗 1棉铃虫的死亡率与中心Bt相近 ,且随播种日期推迟棉铃虫死亡率逐渐升高。试验结果表明 :双抗 1对棉铃虫的抗性水平与中心Bt相近 ,且抗性表现为前期高后期低。也对双价抗虫棉的时空表达特征与棉铃虫产生抗性的风险关系进行了讨论
The Bt + Bt transgenic cotton Bt + cotton cultivar Bt was used as a control to study the spatiotemporal expression of Bt + CpTI double-resistant cotton (Bt + CpTI) against cotton bollworm. During cotton growth and development, the newly hatched larvae of H. armigera were fed on the main stem leaves of 4 ~ 21 leaves. After 5 days of observation, there were no 3-instar larvae in the double-resistant 1 and 4 ~ 2 1 leaves of the central Bt The 3rd instar larvae of Sumian 12 accounted for more than 40% of the surviving larvae. The mortality rates of double-antibody 1 and central Bt bollworm were similar and decreased with leaf position increasing. The staged seeding insect resistance test was similar to the insect resistance test of each leaf position, that is, there was no 3 instar larvae in surviving cotton bollworm at each seeding stage of double-antibody 1 and center Bt, and 3 instar insects occurred at each sowing date of Su-cotton 12, The mortality of double-resistant 1 bollworm was similar to that of central Bt, and the mortality of cotton bollworm gradually increased with the sowing date. The results showed that the resistant level of double-antibody 1 to cotton bollworm was similar to that of central Bt, and its resistance was low in early high-stage. The relationship between the spatiotemporal expression characteristics of bimonthly insect-resistant cotton and the risk of cotton bollworm-resistant was also discussed