论文部分内容阅读
检测输液污染有多种定性和定量方法,本文作者比较倾注培养法、ATP活性测定、鲎变形细胞溶解物(LAL)试验三种方法的敏感性和实用意义。选用7种实验菌株(大肠杆菌、产气杆菌、肺炎杆菌、阴沟杆菌、枸橼酸杆菌等)进行试验:(1)倾注培养:取原液和10~(-1)～10~(-4)稀释液各1.0和0.1ml分别作双份倾注培养,置35～37℃孵育24～48小时,计数菌落,计算每毫升活菌数;(2)ATP活性测定;用生物发光法测定细胞或细胞提取物的ATP活性,直接取原浓度或经Tris缓冲液稀释的样品0.1ml虫荧光素酶(Iuciferase)0.1ml于反应容器内,充分混合,通过发光效应检查以表达ATP活性;(3)LAL试验:用LAL
There are many qualitative and quantitative methods for the detection of transfusion contamination. The author of this study compared the sensitivity and practical significance of three methods: culture method, ATP activity assay and LAL assay. Seven kinds of experimental strains (Escherichia coli, Aerobacter aerogenes, Klebsiella pneumoniae, Enterobacter cloacae, Citrobacter, etc.) were selected for experiment: (1) Pouring culture: Take stock solution and 10-1 to 10-4, 1.0 and 0.1 ml of dilutions were separately doubly poured and incubated at 35-37 ° C for 24-48 hours. Colonies were counted and the number of viable cells per milliliter was calculated. (2) Determination of ATP activity; determination of cells or cells by bioluminescence Extract the ATP activity, the original concentration or the sample diluted with Tris buffer 0.1ml 0.1ml Luciferase (luciferase) in the reaction vessel, well mixed, through the light-emitting effect to express ATP activity; (3) LAL Test: LAL