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目的研究蜥蜴胃康基本方对胃癌前病变(PLGC)模型大鼠缺氧诱导因子HIF-1α和血管内皮生长因子VEGF表达的影响,探讨蜥蜴胃康基本方对PLGC的调控机制。方法将70只SD雄性大鼠随机分成空白对照组、胃癌前病变模型组、蜥蜴胃康基本方全方组、补气组、养阴组、解毒通络组、维酶素对照组。除空白对照组外,余组均采用浓度0.02mmol/L的N-甲基-N’-硝基-N-亚硝基胍(MNNG)灌胃及自由饮用、40%乙醇灌胃、饥饱失常综合因素,共24周来制备胃癌前病变大鼠模型。分别用蜥蜴胃康基本方、补气药、解毒通络药、养阴药、维酶素连续治疗12周,观察大鼠胃黏膜病理组织学变化,应用免疫组化法检测胃体组织HIF-1α和VEGF因子表达情况。结果通过Image-Pro Plus6.0图像分析系统对免疫组化图像进行光密度的测算,各组与模型组比较P<0.05;各治疗组与空白对照组比较P<0.05;蜥蜴胃康基本方全方及补气、解毒通络治疗组HIF-1α、VEGF因子阳性表达率低于维酶素治疗组(P<0.05),其中以全方组AOD值最小;三组拆方比较P<0.05,其中补气组AOD值最小,解毒通络组次之,养阴组表达较高。结论蜥蜴胃康基本方及其拆方能部分逆转PLGC模型大鼠胃黏膜病理变化,降低HIF-1α、VEGF阳性表达,可促进细胞凋亡,抑制细胞过度增殖,这可能是蜥蜴胃康基本方干预PLGC的分子生物学机制。
Objective To study the effect of likankie kang basic recipe on hypoxia inducible factor (HIF-1α) and vascular endothelial growth factor (VEGF) expression in gastric precancerous lesions (PLGC) model rats, and to explore the regulation mechanism of lizards weikang basic formula on PLGC. Methods Seventy SD male rats were randomly divided into blank control group, gastric precancerous lesion model group, lizard Weikangbi Fangfangfang group, Buqi group, Yangyin group, Jiedu Tongluo group and Vitexin control group. Except the blank control group, the remaining group were fed with MNNG 0.02 mmol / L and gavage, 40% ethanol gavage, hungry Abnormal factors, a total of 24 weeks to prepare gastric precancerous rat model. Respectively with lizard stomach Kang basic side, qi medicine, detoxification Tongluo drugs, nourishing Yin drugs, vitamins for 12 weeks continuous observation of gastric mucosal histopathological changes, immunohistochemical detection of gastric tissue HIF- 1α and VEGF factor expression. Results The optical density of the immunohistochemical images was calculated by Image-Pro Plus6.0 image analysis system, P <0.05 in each group compared with the model group; P <0.05 in each treatment group compared with the blank control group; (P <0.05). The AOD values of all the prescriptions were the lowest. The three prescriptions were significantly lower than P <0.05, and the positive rates of HIF-1α and VEGF were lower Among them, the AOD value of qi supplement group was the lowest, followed by that of detoxification and mending group, while that of yin-yin group was the highest. Conclusion Lizhenweikang Decoction can partly reverse the pathological changes of gastric mucosa in rats with PLGC and decrease the positive expression of HIF-1α and VEGF, which can promote cell apoptosis and inhibit the proliferation of cells, which may be the basic form of Lizo Weikang Intervention in molecular biology of PLGC.