研究肿瘤坏死因子（TNF－α）在正常和Graves病（GD）甲状腺生长调节中的作用。方法：采用GD和甲状腺腺瘤旁正常组织，以细胞单层培养及非同位素标记的细胞增殖分析法，检测不同浓度TNF－α对甲状腺细胞生长功能的影响。结果：TNF－α对基础状态下正常甲状腺细胞的生长无明显调节作用，但剂量依赖性地抑制TSH对此类细胞增殖的刺激效应。在10－2～102ng／ml的浓度区间，TNF－α不影响GD甲状腺细胞基础及TSH诱导的生长功能。结论：TNF－α在甲状腺稳态和甲状腺肿大的发病机理中发挥重要作用。 To investigate the role of tumor necrosis factor (TNF-α) in the regulation of thyroid gland growth in normal and Graves’ disease (GD). Methods: The effects of different concentrations of TNF-α on the growth of thyrocytes were detected by using GD and thyroid adenoma adjacent normal tissues. Cell monolayer culture and non-isotope labeled cell proliferation assay were used. Results: TNF-α had no significant effect on the growth of normal thyroid cells under basal condition, but inhibited the stimulatory effect of TSH on the proliferation of these cells in a dose-dependent manner. In the concentration range of 10-2 ~ 102ng / ml, TNF-α did not affect the basis of GD thyroid cells and TSH-induced growth function. Conclusion: TNF-α plays an important role in the pathogenesis of thyroid homeostasis and goiter.