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目的:观察矾冰纳米乳对深Ⅱ度烧伤模型兔NOTCH通路靶基因表达的影响。方法:采用铁筒烫伤的方法,将200只新西兰大白兔造模后,随机分为5组,分别为空白对照组、烫疮油组及矾冰纳米乳低、中、高浓度组,分别于干预后3、7、14、21、28 d处死后取材,用RT-PCR的方法检测NOTCH靶基因Hes、Hey、Herp的表达。结果:不同浓度的矾冰纳米乳及烫疮油组可调控Hes、Herp基因的表达,均优于空白对照组;矾冰纳米乳中浓度组明显优于烫疮油组及矾冰纳米乳低、高浓度组(P<0.05);各组间Hey基因差异无统计学意义(P>0.05)。结论:矾冰纳米乳能调控深Ⅱ度烧伤模型兔NOTCH靶基因Hes、Herp的表达,中浓度的矾冰纳米乳(16.3 mg/m L)为调控NOTCH靶基因Hes、Herp的最优浓度。
Objective: To observe the effect of alum ice nanoemulsion on target gene expression of NOTCH pathway in deep second degree burn model in rabbits. Methods: 200 New Zealand white rabbits were randomly divided into 5 groups, which were blank control group, scald oil group and alum ice nanoemulsion low, medium and high concentration group At 3,7,14,21,28 d after the intervention, the animals were sacrificed and the expression of NOTCH target genes Hes, Hey and Herp were detected by RT-PCR. Results: The expression of Hes and Herp gene in different concentrations of alum ice nanoemulsion and scald oil group was better than that of the blank control group. The concentration of alum ice nanoemulsion group was significantly better than that of the scald oil group and alum ice nanoemulsion group , High concentration group (P <0.05). There was no significant difference in Hey gene among the groups (P> 0.05). CONCLUSION: Alum ice nanoemulsion can regulate the expression of NOTCH target gene Hes and Herp in rabbit model of deep second-degree burn, while the concentration of alum ice nanoemulsion (16.3 mg / mL) is the optimal concentration of NOTCH target gene Hes and Herp.