论文部分内容阅读
采用溴乙锭荧光法测定氧化型染发剂的主要成分对苯二胺（ＰＰＤ）、过氧化氢（Ｈ２Ｏ２）和ＰＰＤ－Ｈ２Ｏ２混合物对小牛胸腺ＤＮＡ的双链间交联形成作用。结果表明：用０～６％的对苯二胺及０～３％的Ｈ２Ｏ２对小牛胸腺ＤＮＡ直接染毒，３７℃３０ｍｉｎ时无双链间ＤＮＡ交链的形成；而ＰＰＤ和Ｈ２Ｏ２发生氧化反应后则具有双链ＤＮＡ交联形成作用并具有剂量反应关系（ｒ＝０．８２９，Ｐ＜０．０１），在受试剂量为０～０．８％（以对苯二胺含量计）时，氧化型对苯二胺的ＤＮＡ交联作用增加了４．２倍。研究结果提示，ＤＮＡ交联作用可能是氧化型染发剂遗传毒作用过程中ＤＮＡ损伤的一种方式。
The main components of the oxidative hair dye were measured by ethidium bromide spectrophotometry. The effect of PPD, hydrogen peroxide (H2O2) and PPD-H2O2 mixture on the double-stranded cross-linking of calf thymus DNA was studied. The results showed that there was no DNA cross-linking between double strands at 37 ℃ for 30min when the calf thymus DNA was directly exposed to 0-6% p-phenylenediamine and 0-3% H2O2. After the oxidation reaction of PPD and H2O2 (R = 0.829, P <0.01). At doses of 0-0.8% (based on p-phenylenediamine content), the double-stranded DNA was cross-linked and formed in a dose- , Oxidized para-phenylenediamine DNA cross-linking increased 4.2-fold. The results suggest that DNA cross-linking may be a way of DNA damage in the genotoxicity of oxidative hair dyes.