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目的探讨hTR反义寡脱氧核苷酸(ASODN)联合顺铂对人胃癌裸鼠皮下移植瘤的治疗作用。方法30只裸鼠建立胃癌皮下移植瘤模型后,随机分成5组,予以不同条件处理,对照组(瘤周注射RPMI1640培养液)、ASODN组(瘤周注射反义寡脱氧核苷酸)、RODN组(瘤周注射随机寡脱氧核苷酸)、DDP组(瘤周注射化疗药物顺铂)、ASODN+DDP组(瘤周注射反义寡脱氧核苷酸和顺铂)。治疗后定期观察肿瘤体积,计算肿瘤抑制率。采用TRAPPCR-ELISA法检测移植瘤的端粒酶活性。结果ASODN+DDP组、ASODN组、DDP组和RODN组的最高肿瘤抑制率分别为94.2%、84.3%,92.8%和26.9%。在端粒酶活性检测中,4个治疗组间均有统计学意义。结论hTR反义PS-ODN对人胃癌裸鼠皮下移植瘤生长及端粒酶活性有一定抑制作用,且可增强DDP的上述作用。
Objective To investigate the therapeutic effect of hTR antisense oligodeoxynucleotide (ASODN) combined with cisplatin on human gastric cancer xenografts in nude mice. Methods Thirty nude mice were randomly divided into 5 groups and treated with different conditions. The control group (peritumoral injection of RPMI1640 medium), ASODN group (peritumoral injection of antisense oligodeoxynucleotide), RODN Group A (Peritoneal injection of random oligodeoxynucleotide), DDP group (Peritoneal injection of cisplatin), ASODN + DDP group (Peritumor injection of antisense oligodeoxynucleotide and cisplatin). After treatment, the tumor volume was observed regularly, and the tumor inhibition rate was calculated. TRAPPCR-ELISA was used to detect the telomerase activity in the xenografted tumor. Results The highest tumor inhibition rates in ASODN + DDP, ASODN, DDP and RODN groups were 94.2%, 84.3%, 92.8% and 26.9%, respectively. In the detection of telomerase activity, the four treatment groups were statistically significant. Conclusion hTR antisense PS-ODN can inhibit the growth and telomerase activity of human gastric cancer xenografts in nude mice, and enhance the above-mentioned effects of DDP.