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目的 观测缺氧预适应的小鼠脑提取液对大鼠海马神经元ATP敏感性钾通道 (KATP)的影响。方法 用膜片钳全细胞记录优降糖 (GLI)处理前后急性分离大鼠海马神经元外向钾电流 (Ik)的变化。结果 给予氰化钠 (NaCN)时 ,Ik显著增加 (14 4 8→ 2 381pA) ,再给予GLI时 ,Ik增加受到抑制 (2 381→ 172 5pA) ;给予腺苷 (ADO)和GLI后 ,Ik的相应显著变化为 1399→ 2 5 84→ 170 3pA ;给予 4次缺氧预适应鼠脑提取液和GLI后 ,Ik亦产生类似的显著变化 (12 98→ 2 4 13→ 1713pA) ;1次缺氧未缺氧鼠脑匀浆提取液对Ik的作用未见显著影响。结论 缺氧预适应小鼠脑提取液 ,通过腺苷样神经活性物质 ,.激活海马神经元KATP,参与缺氧预适应
Objective To observe the effect of hypoxic preconditioning brain extract on ATP - sensitive potassium channel (KATP) in rat hippocampal neurons. Methods Patch clamp whole cells were used to record the changes of outward potassium current (Ik) in hippocampal neurons of acutely isolated rats before and after treatment with glibenclamide (GLI). Results Ik increased significantly (14 4 8 → 2 381 pA) when sodium cyanide (NaCN) was given, whereas inhibition of Ik was inhibited after GLI administration (2 381 → 172 5 pA); after administration of adenosine (ADO) and GLI, Ik The corresponding significant changes were 1399 → 2 5 84 → 170 3pA; Ik also produced similar significant changes (12 98 → 2 4 13 → 1713 pA) after 4 hypoxic preconditioning brain extracts and GLI; Oxygen anaerobic rat brain homogenate extract had no significant effect on Ik. Conclusions The hypoxic preconditioned mouse brain extract can activate KATP of hippocampal neurons through adenosine-like neuroactive substances and participate in hypoxia preconditioning