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目的:探讨精氨酸-甘氨酸-天冬氨酸(RGD)肽涂层微种植体在不同愈合时间时骨组织中Ⅰ型胶原(ColⅠ)和骨钙蛋白(OC)表达水平的变化,阐明RGD肽涂层对微种植体骨整合的促进作用。方法:选取6只成年雄性Beagle犬,将36枚微种植体分为2组,实验组为RGD肽涂层微种植体,对照组微种植体表面未做处理(n=18)。采用随机对照原则,在第0、4、6周时分别在Beagle犬上颌骨左、右侧植入区(第2、第3、第4前磨牙根分叉区域随机选取任一部位)各植入1枚种植体,一侧为实验组,对侧为对照组。实验第8周时处死动物,获得愈合时间为2、4和8周的微种植体-骨组织标本,行HE及免疫组织化学染色,并检测微种植体周围骨组织中ColⅠ和OC的表达情况。结果:HE染色,实验组与对照组均显示有一定的成骨反应,愈合2和4周时,实验组与对照组比较骨组织表现为较强的成骨反应,8周时骨反应趋于一致,均可见板层状骨组织。实验组微种植体周围骨组织中ColⅠ和OC表达水平在2和4周时高于对照组(P<0.05),8周时2组比较差异无统计学意义(P>0.05)。实验组愈合4周时微种植体周围骨组织中ColⅠ表达水平高于2和8周时,且差异有统计学意义(P<0.05)。对照组仅2周与8周时ColⅠ表达水平比较差异有统计学意义(P<0.05)。实验组与对照组微种植体周围骨组织中OC表达水平随愈合时间延长而逐渐升高,8周时表达水平明显高于2周时(P<0.05);实验组愈合4周时OC表达水平明显高于2周时(P<0.05),而对照组2周与4周时比较差异无统计学意义(P>0.05)。结论:RGD肽涂层能够提高微种植体周围骨组织中ColⅠ和OC的表达,其在促进微种植体初期骨整合过程中起重要作用。
OBJECTIVE: To investigate the changes of collagen type Ⅰ (ColⅠ) and osteocalcin (OC) in bone tissue of different concentrations of RGD peptide coated micro-implants at different healing time, and to clarify that RGD Promotion of peptide coating on osseointegration of micro-implants. Methods: Six adult male Beagle dogs were selected and 36 micro-implants were divided into two groups. The experimental group was RGD peptide-coated micro-implants. The surface of micro-implant was not treated in the control group (n = 18). According to the principle of randomized control, the left and right implantation area of the maxillary bone of Beagle dogs were randomly selected at the 0, 4, and 6 weeks after implantation (randomly selected at the second, third and fourth premolar root bifurcation areas) One implant, one side for the experimental group, contralateral to the control group. Animals were sacrificed on the 8th week of the experiment to obtain micro-implant-bone tissue specimens with healing time of 2, 4 and 8 weeks. HE staining and immunohistochemical staining were performed. The expression of ColⅠ and OC in bone tissue around the micro-implants . Results: The HE staining showed that the experimental group and the control group showed some osteogenic reaction. At 2 and 4 weeks after healing, the bone tissue in the experimental group and the control group showed a stronger osteogenic response. At 8 weeks, the bone reaction tended to Consistent, are visible lamellar bone tissue. The expression of ColⅠ and OC in the bone tissue around the micro-implants in the experimental group was higher than that in the control group at 2 and 4 weeks (P <0.05). There was no significant difference between the two groups at 8 weeks (P> 0.05). The expression of ColⅠ in the bone tissue around the micro-implant was higher than that in the experimental group at 2 and 8 weeks after healing for 4 weeks, and the difference was statistically significant (P <0.05). The expression of Col Ⅰ in the control group only had significant difference at 2 weeks and 8 weeks (P <0.05). The expression level of OC in the bone tissue around the micro-implants increased gradually with the prolongation of healing time in experimental group and control group, and the expression level of OC in the experimental group and the control group was significantly higher than that in the second week (P <0.05) (P <0.05), but there was no significant difference between two weeks and four weeks in the control group (P> 0.05). Conclusion: RGD peptide coating can increase the expression of ColⅠ and OC in bone tissue around micro-implants, which plays an important role in promoting the early osseointegration of micro-implants.