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目的探讨褪黑素对小鼠动态吸入火箭煤油肺组织氧化损伤的防护。方法选择雄性ICR小鼠18只,随机分为正常对照组、火箭煤油组,褪黑素干预组,每组6只。吸入染毒前2 h,褪黑素干预组灌胃2 mg/kg的褪黑素,火箭煤油组灌胃0.5%羧甲基纤维素溶液。所有小鼠分别置于防爆式动态吸入染毒柜中进行吸入染毒,火箭煤油染毒组和褪黑素干预组分别通以火箭煤油(设染浓度为18 000 mg/m~3),正常对照组通以室外洁净空气,小鼠动态吸入染毒4 h,染毒后2 h测定肺组织中TAOC、SOD和GSH-Px的活性和MDA的含量。结果与正常对照组比较MDA:(0.98±0.15)nmol/mg Pr;TAOC:(1.33±0.11)U/mg Pr;SOD:(5.21±0.38)U/mg Pr;GSH-Px:(73.26±12.69)U/mg Pr,染毒后2 h吸入火箭煤油组小鼠肺组织MDA(1.18±0.04)nmol/mg Pr的含量显著增加(P<0.05),TAOC(1.19±0.10)U/mg Pr、SOD(4.40±0.29)U/mg Pr和GSH-PX(56.83±9.70)U/mg Pr的活性显著降低(P<0.05)。褪黑素能明显降低火箭煤油组小鼠肺组织中MDA的含量(1.07±0.11)nmol/mg Pr,P<0.05,显著增加TAOC(1.29±0.06)U/mg Pr、SOD(4.93±0.44)U/mg Pr和GSH-Px(67.17±3.67)U/mg Pr的活性,P<0.05。结论褪黑素能减轻吸入火箭煤油诱导的小鼠肺组织氧化损伤,对肺组织有一定的保护作用。
Objective To investigate the protective effect of melatonin on lung injury induced by rocket kerosene in rats. Methods 18 male ICR mice were randomly divided into normal control group, rocket kerosene group and melatonin intervention group, with 6 rats in each group. Two hours before inhalation, melatonin intervention group was given intragastric administration of 2 mg / kg of melatonin, and rocket kerosene group was given 0.5% carboxymethylcellulose solution. All mice were placed in an explosion-proof dynamic inhalation cabinet for inhalation exposure, rocket kerosene exposure group and melatonin intervention group were rocket kerosene (set for the concentration of 18 000 mg / m ~ 3), normal The control group was exposed to clean air outdoors. The mice were challenged by dynamic inhalation for 4 h and the activities of TAOC, SOD and GSH-Px and the contents of MDA in the lung tissue were determined 2 h after exposure. Results Compared with the normal control group, MDA: (0.98 ± 0.15) nmol / mg Pr; TAOC: (1.33 ± 0.11) U / mg Pr; SOD: (5.21 ± 0.38) U / mg Pr; GSH-Px: (73.26 ± 12.69 ) U / mg Pr. The contents of MDA (1.18 ± 0.04) nmol / mg Pr were significantly increased (P <0.05), TAOC (1.19 ± 0.10) U / mg Pr, The activities of SOD (4.40 ± 0.29) U / mg Pr and GSH-PX (56.83 ± 9.70) U / mg Pr were significantly decreased (P <0.05). Melatonin significantly reduced the content of MDA (P <0.05) and significantly increased the TAOC (1.29 ± 0.06) U / mg Pr and SOD (4.93 ± 0.44) in the lung tissue of rocket kerosene- U / mg Pr and GSH-Px (67.17 ± 3.67) U / mg Pr, P <0.05. Conclusion Melatonin can reduce the oxidative damage of lung tissue induced by inhaled rocket kerosene in mice, and has certain protective effect on lung tissue.