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目的观察转人乳铁蛋白基因牛奶粉(LF)有无致突变效应。方法将40只雄性昆明小鼠随机分为8组,以2 500、5 000、10 000 mg/kg的转人乳铁蛋白基因牛奶粉(LF)连续灌胃5 d,以同等剂量的普通奶粉(荷斯坦奶牛)为亲本对照,并设阴性对照组和阳性对照组。于小鼠首次染毒后第35天观察精子畸形率。将80只昆明种小鼠按上述剂量及分组方法分为8组。间隔24 h两次给小鼠经口灌胃,观察骨髓嗜多染红细胞微核率。采用鼠伤寒沙门菌组氨酸缺陷型TA97、TA98、TA100、TA102四株试验菌株,LF设0.2、0.5、1.0、2.5、5.0 mg/皿5个剂量,以同等剂量的普通奶粉(荷斯坦奶牛)为亲本对照,并设阴性对照组和阳性对照组,采取非代谢活化和代谢活化(加S9)两种方式进行Ames试验。结果转人乳铁蛋白基因牛奶粉(LF)小鼠精子畸形试验、小鼠骨髓细胞微核试验、Ames试验结果均为阴性。结论在本试验条件下未见转人乳铁蛋白基因牛奶粉(LF)有致突变效应。
Objective To observe the mutagenic effects of lactoferrin gene transfer milk powder (LF). Methods Forty male Kunming mice were randomly divided into 8 groups, fed continuous lactoferrin milk powder (LF) at a dose of 2 500, 5 000 and 10 000 mg / kg for 5 days. The same dose of ordinary milk powder (Holstein cows) as the parental control, and set negative control group and positive control group. Sperm deformity was observed on the 35th day after the mice were first exposed. Eighty Kunming mice were divided into 8 groups according to the above dosage and grouping method. The mice were orally gavaged twice daily for 24 h to observe the micronuclei rate of bone marrow polychromatic erythrocytes. Four test strains, TA97, TA98, TA100 and TA102 of Salmonella typhimurium were used. Five doses of 0.2, 0.5, 1.0, 2.5 and 5.0 mg / ) For the parental control, and set negative control group and positive control group, to take non-metabolic activation and metabolic activation (plus S9) Ames test two ways. Results The results of sperm deformity test, micronucleus test of bone marrow cells and Ames test in mice fed lactoferrin gene were all negative. Conclusion No mutagenic effects of lactoferrin gene transfer milk powder (LF) were observed under the experimental conditions.