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采用微茎尖组培技术可以大量繁育香石竹的无病毒生产用苗株,在增殖培养中选用不加激素的MS培养基、小容积容器及透气封口膜处理容器瓶口,均可有效地降低培养过程中的玻璃化株率;生根培养基的配方以MS+NAA0.05mg/L为最适用,香石竹组培生根苗的繁育系数9个月可以达到1167倍。本试验以指示植物苋色藜汁液磨擦接种法鉴定所繁育的香石竹苗有无环斑、斑驳病毒。
The use of micro-tip tissue culture technology can be a large number of breeding carnation plant virus-free production of seedlings in the proliferation of culture medium without hormone selection of MS medium, small volume containers and breathable sealing film processing container bottle, can effectively reduce The rate of vitrification in the process of culture was the best. The best rooting medium was MS + NAA 0.05 mg / L, the breeding coefficient of root sprout of carnation was 967 months. This experiment to indicate the plant amaranth quinoa juice friction inoculation method identified by the breeding of carnation seedlings have no ring spot, mottle virus.