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目的探讨鼠疫耶尔森菌(鼠疫菌)基因分型在鼠疫暴发中的流行病学意义。方法根据已经证实的23个差异区段设计引物,对2004年青海省人间鼠疫流行期间分离到的13株鼠疫菌进行PCR扩增。结果 13株鼠疫菌可分为4个基因组型,即Genomovar 8、10、15和16。其中囊谦的8 株菌全部为Genomovar 10;乌兰的2株菌分别为Genomovar 8和15;祁连、曲麻莱、称多的3株鼠疫菌的基因组型均为Genomovar 16。结论鼠疫菌基因分型是鼠疫疫情暴发流行病学调查的有力工具。
Objective To investigate the epidemiological significance of Yersinia pestis (Yersinia pestis) genotyping in outbreaks of plague. Methods Based on the 23 differentially identified segments, the 13 strains of Yersinia pestis isolated during the epidemic of human plague in Qinghai Province in 2004 were amplified by PCR. Results 13 strains of Y. pestis were divided into 4 genotypes, Genomovar 8, 10, 15 and 16. Among them, the eight strains were all Genomovar 10; the two strains of Ulan were Genomovar 8 and 15; the genotypes of three strains of Yersinia pestis Qilian and Qumalai were all Genomovar 16. Conclusion Yersinia pestis genotyping is a powerful tool for epidemiological investigation of plague outbreaks.