论文部分内容阅读
为发掘影响水稻产量性状的有利等位变异及其载体材料用于品种产量潜力改良,2011和2012年调查了不同地理来源的540个亚洲栽培稻品种的4个产量性状,鉴定了这540个品种262对SSR标记的基因型,利用STRUCTURE 2.2软件分析了群体结构,运用TASSEL 2.1软件中的一般线性模型检测了与4个性状显著关联的SSR标记位点。结果表明:540个品种组成的自然群体中单株有效穗数、每穗实粒数、千粒质量和单株籽粒产量的变异系数值2年平均分别为36.73%、29.90%、10.08%和35.21%。自然群体被划分为7个亚群。各亚群内均存在不同程度的连锁不平衡。2年均检测到的与4个性状显著关联的SSR标记位点有30个。其中与单株有效穗数显著关联的有9个,携有RM3533-105等位变异的‘晚芦栗’对增加单株有效穗数效应最大;与每穗实粒数显著关联的有11个,携有RM5746-120等位变异的‘越108’对增加每穗实粒数效应最大;与千粒质量显著关联的有6个,携有RM17-145等位变异的‘宁粳恢338’对增加千粒质量效应最大;与单株籽粒产量显著关联的有4个,携有RM206-245等位变异的‘老来红’对增加单株籽粒产量效应最大。结论:不同地理来源的水稻品种含有控制不同性状的最优等位变异,可用于不同目标性状的改良。
In order to explore favorable allelic variations affecting rice yield traits and their vector materials for yield improvement, 4 yield traits of 540 Asian cultivated rice cultivars from different geographical sources were investigated in 2011 and 2012, and 540 cultivars 262 pairs of SSR markers were genotyped, population structure was analyzed using STRUCTURE 2.2 software and SSR markers significantly associated with the four traits were detected using the general linear model in TASSEL 2.1 software. The results showed that the coefficient of variation (CV) of effective panicle, panicles per panicle, grain yield per panicle and grain yield per plant were 36.73%, 29.90%, 10.08% and 35.21 in the natural population of 540 cultivars, respectively %. Natural groups are divided into seven subgroups. Each subgroup there are varying degrees of linkage disequilibrium. There were 30 SSR markers that were significantly associated with 4 traits in 2 years. Among them, 9 were significantly correlated with the effective panicle per plant, and the effect of ’Ulmus pumila’ with the allelic variation of RM3533-105 on the effective panicle number per plant was the highest. There were 11 , The more grain 108 with the allelic variation of RM5746-120 had the strongest effect on the grain number per panicle; 6 were significantly associated with the 1000 grain weight, and Ning Jing Hui 338 with the allelic variation of RM17-145 The effect of increasing grain weight per plant was the highest. Four plants were significantly associated with grain yield per plant, and the effect of ’Laolaihong’ bearing the allelic variation of RM206-245 on the yield per plant was greatest. CONCLUSION: Rice varieties from different geographical origins contain the optimal allelic variation for controlling different traits and can be used for improvement of different target traits.