论文部分内容阅读
目的观察维生素B_2对急性低氧暴露小鼠血浆代谢组的改善作用,探讨可能的机制。方法将雄性昆明小鼠随机分为:正常对照组、低氧对照组及2倍、4倍和8倍供给量的维生素B_2补充组,以相应饲料喂养2周后,除正常对照组外,其他各组均模拟6000m高度停留8h,采集血浆,以核磁共振的方法分析其代谢组变化。结果急性低氧暴露后,血浆代谢组发生广泛变化;模式识别分析结果显示,各组血浆代谢组在得分图中呈聚类型分布,且有先分离后回归的代谢模式变化轨迹,显示出补充不同剂量维生素B_2后小鼠血浆代谢组逐渐恢复的趋势;各组血浆代谢组产生差别的原因是脂类、乳酸、丙氨酸、N-乙酰糖蛋白、谷氨酸、胆碱、牛磺酸、糖、肉碱、甘氨酸、肌酐等物质的水平发生变化,表明相关的代谢途径发生了变化。结论急性低氧暴露导致机体营养代谢发生显著变化,补充维生素B_2可改善急性低氧暴露机体碳水化合物和脂肪代谢,其部分作用可能通过肉碱介导实现。
Objective To observe the effect of vitamin B 2 on plasma metabolism in acute hypoxic exposure mice and to explore the possible mechanism. Methods Male Kunming mice were randomly divided into two groups: normal control group, hypoxia control group and 2-fold, 4-fold and 8-fold supplemented groups of vitamin B 2 supplemented with corresponding feed for 2 weeks, except for the normal control group Each group were simulated at a height of 6000m for 8h, plasma was collected, and their metabolome changes were analyzed by nuclear magnetic resonance. Results After acute hypoxic exposure, the plasma metabolome changed widely. The results of pattern recognition showed that the plasma metabolome of each group showed clustered distribution in the score graph, and there was a trace of the metabolic pattern of regression after the first separation, indicating different supplementation Dose of vitamin B 2 mice plasma metabolome gradually recovery trend; plasma metabolites of each group differences are due to lipids, lactate, alanine, N-acetylglucosamine, glutamic acid, choline, taurine, Carnitine, carnitine, glycine, creatinine and other substances change, indicating that the relevant metabolic pathways have changed. Conclusion Acute hypoxic exposure leads to significant changes in nutritional metabolism. Vitamin B 2 supplementation can improve carbohydrate and fat metabolism in acute hypoxia exposure and some of its effects may be mediated by carnitine.