基因治疗的必要条件是:①对有关导入基因及其产物的知识有详尽的了解;②外来基因能有效地导入靶细胞;③导入基因能在靶细胞中长期稳定驻留;④导入基因能有适度水平的表达;⑤基因导入的方法及所用载体对宿主细胞安全无害。以逆转录病毒作为载体进行基因治疗的研究较多,但目前开发逆转录病毒以外的载体的工作也正 The necessary conditions for gene therapy are: (1) a detailed understanding of the knowledge about the introduced genes and their products; (2) the foreign genes can be efficiently introduced into the target cells; (3) the introduced genes can stably stay in the target cells for a long time; (4) Moderate level of expression; ⑤ gene transfer method and the vector used for the host cell safe and harmless. There is a lot of research on gene therapy using retrovirus as a vector, but at present, the work of developing vectors other than retroviruses