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目的:建立帕罗醇中反式帕罗醇和顺式帕罗醇的含量测定方法。方法:色谱柱为Phenomenex硅胶柱(250mm×4.6mm,5μm),以正己烷-乙醇-二乙胺(68:32:0.5)为流动相,流速为1.0ml·min~(-1),检测波长为267nm,柱温30℃。结果:反式帕罗醇、顺式帕罗醇与其他杂质之间能够达到良好分离;反式帕罗醇在5.0～4000.0μg·ml~(-1)浓度范围内线性关系良好(r=0.9999);顺式帕罗醇在2.0～800.0μg·ml~(-1)浓度范围内线性关系良好(r=0.999 9);反式帕罗醇和顺式帕罗醇平均加样回收率分别为99.60%、98.73%,RSD分别为0.61%、0.94%。结论:该法能够分别同时准确测定帕罗醇中两个异构体的含量,而且简便、准确、快速,可用于样品的质量控制。
Objective: To establish a method for the determination of trans-parvalol and cis-palnaol in parol. METHODS: The chromatographic column was a Phenomenex silica gel column (250 mm × 4.6 mm, 5 μm) with a mobile phase of n-hexane-ethanol-diethylamine (68:32:55) at a flow rate of 1.0 ml · min -1 Wavelength of 267nm, column temperature 30 ℃. Results: The trans-parvalcohol and cisplatinol could be well separated from other impurities. The trans-parvalcohol showed good linearity in the range of 5.0-4000.0μg · ml -1 (r = 0.9999 ) Showed good linearity (r = 0.999 9) for cisplatinol in the concentration range of 2.0 ~ 800.0μg · ml ~ (-1); mean recovery rates of trans-paltophanol and cisplatin were 99.60 %, 98.73%, RSD 0.61%, 0.94% respectively. Conclusion: The method can simultaneously and accurately determine the content of two isomers of parol, and is simple, accurate and rapid and can be used for the quality control of samples.