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以糯稻H1701为材料,通过胚培养诱导愈伤组织,继代培养并分化.实验结果表明;NaCl对胚性愈伤组织的诱导和生长有不同程度的抑制作用.NaCl浓度越高,抑制越明显.转入分化培养基以后,除含10g/L及以上浓度NaCl的诱导培养基中愈伤组织分化为零外,其余均能分化出苗.其中.含NaCl2g/L的诱导培养基中诱导出的愈伤组织分化率最高.
Glutinous rice H1701 was used as material to induce callus through embryo culture, subculture and differentiation. The experimental results showed that NaCl had different degree of inhibition on the induction and growth of embryogenic callus. The higher the NaCl concentration, the more obvious the inhibition. After transferred into the differentiation medium, all the callus could differentiate in vitro except that the callus in the induction medium containing 10g / L NaCl and above had differentiated into zero. among them. The induction rate of callus in NaCl2g / L induction medium was the highest.