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目的建立一种简便快捷的沙门菌H抗原诱导方法,用于沙门菌的血清分型。方法将沙门菌接种于M肉汤过夜培养进行鞭毛诱导,吸取少量再次进行M肉汤2次诱导,取诱导后的肉汤进行沙门菌血清型鉴定,同时用血平板-滤纸条搭桥法和软琼脂法比对。结果 30株沙门菌同时用3种不同的方法诱导,应用M肉汤法一般1~2次都能鉴定出来,效果比搭桥法和软琼脂法好。应用M肉汤法对369株沙门菌进行血清分型,诱导1次的分型率为81.30%,诱导两次的分型率为97.02%。结论 M肉汤法操作简单、方便、成本低,诱导效果好,适合用于沙门菌血清分型鉴定。
Objective To establish a simple and quick method for induction of Salmonella H antigen for the serotyping of Salmonella. Methods Salmonella was inoculated into M broth overnight to induce flagella. After a small amount of M Broth was inoculated twice, the induced broth was identified for Salmonella serotypes. At the same time, Soft agar than the right. Results Thirty Salmonella strains were induced by three different methods at the same time. The M broth method was generally used one to two times, which was better than the bypass method and the soft agar method. Serotypes of 369 strains of Salmonella were detected by M broth method. The typing rate of the first time was 81.30%, and the rate of the second induction was 97.02%. Conclusion M broth method is simple, convenient, low cost, good induction effect, suitable for Salmonella serotyping identification.