论文部分内容阅读
基于琼脂半固体培养法和极限稀释微孔池培养法,建立了一种新的体外药敏检测体系——微量液体克隆形成法。并应用该体系检测了三尖杉酯碱(H)和高三尖杉酯碱(HH)对CFU—GM及L—CFU的细胞毒作用。结果提示:24h及168h作用法时,HH对CFU—GM以及L—CFU的细胞毒作用均大于H,H、HH对L—CFO的细胞毒作用选择性均大于CFU—GM,但HH的细胞毒作用选择性不及H;H、HH的TDI(Time—schedule Depne—dence Index),IC_(90-24h)÷IC_(-90-168h)分别为68、60,说明两药物均为细胞周期时相特异性药物;并对H、HH之间可能存在的同系物间的交叉耐药性作了探讨。
Based on the agar semi-solid culture method and the limit dilution microporous cell culture method, a new in vitro drug susceptibility detection system-micro-liquid clone formation method was established. The cytotoxic effect of harringtonine (H) and homoharringtonine (HH) on CFU-GM and L-CFU was also tested using this system. The results showed that the cytotoxic effects of HH on CFU-GM and L-CFU were greater than those of H, H and HH at 24h and 168h, but the cytotoxicity of HH to L-CFO was greater than that of CFU-GM. However, Toxicity selectivity less than H; H, HH TDI (Time-schedule Depne-dence Index), IC_ (90-24h) ÷ IC _ (- 90-168h) were 68,60, respectively, indicating that the two drugs are the cell cycle Phase specific drugs; and cross-resistance between H, HH homologues may be explored.