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目的:普查山东省动物源大肠杆菌多重抗药性现状,检测多重抗药性的遗传稳定性。方法:用微量肉汤稀释法检测712株大肠杆菌对15种抗生素的MIC;选取14株多重抗药菌株在MIC/2的各种抗生素中培养,将异常形态菌体接种至半固体LB培养基上,观察其再生能力;同时在无药培养基中传代8次,比较MIC的变化。结果:肉鸡场和病人大肠杆菌的抗药性最为严重,猪场次之,奶牛场最低。多数菌株对5到14种抗生素有抗性。在无药培养基中,丝状菌体能形成新的菌落。消除药物后,35%的菌株抗药性程度降低,甚至转为敏感。结论:细菌抗药性,尤其多重抗药性越来越普遍,对于稳定的抗药性突变可以更换敏感药物,而对于不能稳定遗传的抗药性,间歇用药或限制用药可以保障治疗效果。
Objective: To investigate the status of multiple drug resistance of Escherichia coli in Shandong province and to test the genetic stability of multiple drug resistance. Methods: MIC of 712 Escherichia coli strains against 15 antibiotics was detected by the broth microdilution method. Fourteen multi-drug resistant strains were selected and cultured in various antibiotics of MIC / 2. The abnormal morphogenic bacteria were inoculated into semi-solid LB medium On the observation of its regenerative capacity; at the same time in the drug-free medium eight times, compared MIC changes. Results: The resistance of Escherichia coli in broilers and patients was the most serious, followed by pig farms and lowest in dairy farms. Most strains are resistant to 5 to 14 antibiotics. In drug-free medium, filamentous bacteria can form new colonies. After the drug was eliminated, 35% of the strains were less resistant and even sensitive. CONCLUSIONS: Bacterial drug resistance, especially multi-drug resistance, is becoming more and more common. Sensitive drugs can be replaced for stable drug resistance mutations. However, drug resistance, intermittent drug use or drug restriction can not be stably inherited.