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目的研究毒黄素不同浓度和不同作用时间对非小细胞肺癌A549细胞增殖、凋亡和迁移的影响。方法分别取0.5、0.375、0.25和0.125μmol/L毒黄素作用于A549细胞(实验组),以不加毒黄素为对照组,分别培养24 h和48 h。采用CCK-8试剂盒、Annexin V-FITC/PI凋亡试剂盒检测A549细胞增殖抑制率和凋亡率。划痕实验对比0.125μmol/L毒黄素组和对照组(0μmol/L毒黄素)在12、24和48 h细胞迁移率。结果 0.5、0.375、0.25和0.125μmol/L毒黄素组24 h细胞增殖抑制率分别为(93.51±3.69)%、(40.38±3.08)%、(23.54±2.58)%和(13.07±2.37)%,48 h为(90.53±3.58)%、(53.72±3.02)%、(34.44±3.10)%和(24.78±2.43)%。0.5、0.375、0.25和0.125μmol/L毒黄素组24 h细胞凋亡率分别为(78.68±2.22)%、(43.66±2.53)%、(20.81±2.59)%和(6.25±0.96)%,高于对照组的(1.57±0.52)%;0.5、0.375、0.25和0.125μmol/L毒黄素组48 h细胞凋亡率分别为(88.66±3.16)%、(59.86±2.81)%、(27.89±3.48)%和(9.91±1.33)%,高于对照组的(1.59±0.55)%。0.125μmol/L毒黄素组12、24和48 h细胞迁移率分别为7%、11%和16%,低于对照组相应的14%、26%和39%。结论毒黄素对A549细胞有明显增殖抑制和促凋亡作用,并呈时间和剂量依赖性,且可抑制A549细胞迁移活性。
Objective To study the effects of different concentrations of riboxin and different time on the proliferation, apoptosis and migration of non-small cell lung cancer A549 cells. Methods A549 cells were treated with 0.5,0.375,0.25 and 0.125μmol / L of toxanthin (experimental group). The control group was treated with 24 hours and 48 hours respectively. The proliferation inhibition rate and apoptosis rate of A549 cells were detected by CCK-8 kit and Annexin V-FITC / PI apoptosis kit. Scratch experiments compared the cell migration rates of the 0.125μmol / L toxin group and the control group (0μmol / L toxin) at 12, 24 and 48 hours. Results The inhibitory rates of cell proliferation in the groups of 0.5, 0.375, 0.25 and 0.125 μmol / L for 24 h were (93.51 ± 3.69)%, (40.38 ± 3.08)%, (23.54 ± 2.58)% and (13.07 ± 2.37)%, 48h was (90.53 ± 3.58)%, (53.72 ± 3.02)%, (34.44 ± 3.10)% and (24.78 ± 2.43)%, respectively. The apoptotic rate of 24 h in 0.5, 0.375, 0.25 and 0.125 μmol / L groups was (78.68 ± 2.22)%, (43.66 ± 2.53)%, (20.81 ± 2.59)% and (6.25 ± 0.96)%, (1.57 ± 0.52)% higher than that of the control group; (88.66 ± 3.16)%, (59.86 ± 2.81)%, (27.89%) respectively at 48h after exposure to 0.5,0.375,0.25 and 0.125μmol / ± 3.48)% and (9.91 ± 1.33)%, respectively, higher than that of the control group (1.59 ± 0.55)%. At 12, 24, and 48 h, the cell migration rates in the 0.125 μmol / L toxin group were 7%, 11% and 16%, respectively, which were lower than those of the control group by 14%, 26% and 39%, respectively. Conclusion Toxin A549 can inhibit proliferation and induce apoptosis of A549 cells in a time and dose-dependent manner, and inhibit the migration of A549 cells.