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用~(60)Coγ射线辐照小麦幼胚愈伤组织,可使愈伤组织分化成苗率达50%左右,适宜剂量为1~1.5krad。利用温室加代,在工年时间内可获得γR_0、γR_1两代种子,γR_0代长势弱,出现植株矮化、穗变小及不育株的变异,但基本不遗传,仅有少数穗形、壳色、芒性、籽粒等变异可遗传。γR_1代株高、穗形、壳色、芒性及籽粒性状都有较大变异,总变异率48%~65%,而未经辐照处理的对照,γR_1代的总变异率为32%~38%。γR_1代是选育优良单株的主要世代。γR_1代的变异性状在γR_2代中有80%趋于稳定,γR_2是选育优良无性系的较好世代。同工酶分析表明,各优良无性系与亲本相比都有其特征酯酶带。
Radiation of wheat immature embryos with ~ (60) Co γ-rays can differentiate the callus into about 50% of the seedlings and the suitable dosage is 1 ~ 1.5krad. The generation of seeds of γR_0 and γR_1 can be obtained in the greenhouse during the year of processing, and the growth of γR_0 generation is weak. The emergence of plant dwarf, spikelets and sterile plants are variant, but they are not inherited at all. Only a few spikes and shells Color, malleability, grain variation can be inherited. The variation of plant height, panicle, shell color, mango and grain traits of γR_1 had a great variation, with a total variation rate of 48% -65%. The total variation rate of γR_1 generation was 32% ~ 38%. The γR_1 generation is the major generation of fine plants for breeding. In the γR_1 generation, 80% of the γR_2 generation tended to be stable, and γR_2 was the better generation of the elite clone. Isozyme analysis showed that all elite clones had their characteristic esterase bands compared with their parents.