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以关苍术为试材,通过挂牌调查、扫描电镜观察及花粉培养等方法,研究了关苍术开花动态、花蕊形态及花粉活力。结果表明:关苍术单株花序的开放顺序是自上而下,花序为由外至内,雌花花期为12~14d,两性花花期为14~17d;关苍术花粉最长轴为57.95±2.44μm,属于大孢粉,花粉粒形态指数为1.19,属于长球形;检测花粉活力的染色法中以联苯胺-过氧化氢染色为最好,适宜的花粉培养条件为蔗糖浓度15%、培养温度25℃、培养时间4h,此条件下花粉萌发率达80.4%。
Taking Guan herb as test material, flowering dynamics, flower morphology and pollen viability were studied through the methods of listing investigation, scanning electron microscopy and pollen culture. The results showed that the order of opening of single plant was as follows: the inflorescence was from outside to inside, the flowering period was 12-14 days, the flowering stage was 14-17 days; the longest axis of pollen was 57.95 ± 2.44μm , Belonging to the megaspore powder pollen grain morphology index of 1.19, is a long sphere; pollen vitality staining method to benzidine - hydrogen peroxide staining is the best, suitable pollen culture conditions sucrose concentration of 15%, culture temperature 25 ℃, culture time 4h, pollen germination rate under this condition reached 80.4%.