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目的获得华细辛Asarum sieboldii转录组数据库和差异表达基因,识别华细辛甲基丁香酚生物合成相关基因。方法以华细辛地下部分(根)和地上部分(叶片)2个样本作为供试材料,采用Illumina Hiseq 4000进行转录组测序,并从头拼接,对Unigene进行功能注释和差异性分析。结果共获得12.25 Gb数据,拼接得到129 003个Unigene,可归类于52个GO分类,涉及363条KEGG代谢通路。分析发现,共有439个差异表达基因,其中上调基因占38.3%,下调基因占61.7%,差异较大;136个Unigene与苯丙素类次生代谢途径相关,其中44个Unigene参与甲基丁香酚的生物合成过程。结论本研究得到大量华细辛转录本信息,所发掘的与甲基丁香酚生物合成相关的Unigene为相关基因的分离、功能验证及其表达调控机制的阐明提供了重要依据。
OBJECTIVE To obtain the Asarum sieboldii transcriptome database and differentially expressed genes and to identify the genes involved in the biosynthesis of asarum-methyl-eugenol. Methods Two samples of underground part (root) and aerial part (leaf) of Asarum were used as test materials, sequenced by Illumina Hiseq 4000 and spliced to make functional annotation and difference analysis on Unigene. Results 12.25 Gb data were obtained and 129 003 Unigene were spliced, which could be classified into 52 GO categories involving 363 KEGG metabolic pathways. A total of 439 differentially expressed genes were found, of which 38.3% were upregulated and 61.7% downregulated, with a significant difference; 136 Unigene were associated with the secondary metabolites of phenylpropanoids, of which 44 Unigene were involved in methyl eugenol Biosynthesis process. Conclusions A large number of Asarum transcripts were obtained in this study. Unigene was found to be related to the biosynthesis of methyl eugenol, which provided an important basis for the isolation of related genes, functional verification and elucidation of their regulatory mechanisms.