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[Objective] To screen out the optimal medium for tissue culture of wild Lilium lancifolium.[Method]The tissue culture of wild Lilium lancifolium was studied using its scales,bulbils,stem pieces and leaves as explants respectively.The basic mediums were MS,B5 and White that included 0.03 mg/L of NAA.Four differential mediums were:MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10 mg/L KT+0.10 mg/L NAA and MS+0.03 mg/L NAA.Six proliferation formulas were as below:MS+0.03 mg/L NAA,MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10 mg/L KT,MS+0.20 mg/L KT+0.20 mg/L NAA and MS+0.10 mg/L KT+0.15 mg/L NAA.Four rooting media were:MS+1.00 mg/L IBA,MS+1.00 mg/L IBA+0.20 mg/L 6-BA,1/2MS+1.00 mg/L IBA+0.20 mg/L 6-BA and White+1.00 mg/L IBA+0.20 mg/L 6-BA.[Result] MS medium was the optimum basic medium.The optimal differential mediums for scale,bulbils,leaf and stem pieces of wild Lilium lancifolium were MS+0.03 mg/L NAA,MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L IAA,and MS+2.00 mg/L 6-BA+0.20 mg/L NAA,respectively.The optimum subculture medium was MS+ 0.10 mg/L KT +0.15 mg/L NAA and the optimal medium for rooting of rootless bulbs and seedlings was 1/2MS+0.20 mg/L 6-BA+1.00 mg/L IBA.In the field,the tissue culture seedlings formed by scales and bulbils grew more strongly than others.[Conclusion] The optimal tissue culture mediums for wild Lilium lancifolium were obtained in this work,and this study provided basis for the rapid propagation of wild Lilium lancifolium.
[Objective] To screen out the optimal medium for tissue culture of wild Lilium lancifolium. [Method] The tissue culture of wild Lilium lancifolium was studied using its scales, bulbils, stem pieces and leaves as explants respectively. The basic mediums were MS, B5 and White that included 0.03 mg / L of NAA.Four differential mediums were: MS + 2.00 mg / L 6-BA + 0.20 mg / L NAA, MS + 0.20 mg / L 6-BA + 1.00 mg / L IAA, MS + 0.10 mg / L KT + 0.10 mg / L NAA and MS + 0.03 mg / L NAA.Six proliferation formulas were below: MS + 0.03 mg / L NAA, MS + 2.00 mg / L 6-BA + 0.20 mg / L NAA , MS + 0.20 mg / L 6-BA + 1.00 mg / L IAA, MS + 0.10 mg / L KT, MS + 0.20 mg / L KT + 0.20 mg / L NAA and MS + 0.10 mg / L KT + 0.15 mg / L NAA.Four rooting media were: MS + 1.00 mg / L IBA, MS + 1.00 mg / L IBA + 0.20 mg / L 6-BA, 1/2 MS + 1.00 mg / L IBA + 0.20 mg / L 6-BA and White + 1.00 mg / L IBA + 0.20 mg / L 6-BA. [Result] MS medium was the optimum basic medium. The optimal differential mediums for scale, bulbils, leaf and stem pieces of wild Lilium lancifolium were MS + 0.03 mg / L NAA, MS + 2.00 mg / L 6-BA + 0.20 mg / L NAA, MS + 0 .20 mg / L IAA, and MS + 2.00 mg / L 6-BA + 0.20 mg / L NAA, respectively. The contributing subculture medium was MS + 0.10 mg / L KT + 0.15 mg / L NAA and the optimal medium for rooting of rootless bulbs and seedlings was 1 / 2MS + 0.20 mg / L 6-BA + 1.00 mg / L IBA. In the field, the tissue culture seedlings formed by scales and bulbils grew more strongly than others. [Conclusion] The optimal tissue culture mediums for wild Lilium lancifolium were obtained in this work, and this study provided basis for the rapid propagation of wild Lilium lancifolium.