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本文以番茄为材料,研究H2O2和MAPK在BR诱导的抗氧化防护系统中的作用。结果表明,外源BR提高抗氧化防护酶SOD和CAT活性;而这种诱导机制被H2O2产生抑制剂二苯基碘(DPI)和MEK1/2专一抑制剂PD98059阻断。进一步研究发现:BR能够诱导细胞质外体H2O2的产生,这种诱导被PD98059抑制;BR能够活化一种49kDaMAPK,这种活化被DPI抑制。本研究结果证实细胞质外体H2O2和MAPK候选激酶(49kDaMAPK)信号参与BR诱导的抗氧化防酶途径,且两者之间存在交互作用。
In this paper, we use tomato as material to study the role of H2O2 and MAPK in BR-induced antioxidant protection system. The results showed that exogenous BR increased the activity of antioxidant enzymes SOD and CAT. However, this induction mechanism was blocked by diphenyliodide (DPI) and MEK1 / 2 specific inhibitor PD98059. Further study found that: BR can induce the generation of extracellular body H2O2, this induction is inhibited by PD98059; BR can activate a 49kDaMAPK, this activation is DPI inhibition. The results of this study confirmed that cytoplasmic extracellular body H2O2 and MAPK candidate kinase (49kDaMAPK) signals involved in BR-induced antioxidant enzyme pathway, and there is an interaction between the two.