目的:建立不同产地加工方法浙贝母的高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)的指纹图谱,并进行成分分析和鉴别。方法:液相采用Agilent Eclipse plus C18(4.6mm×250mm,5.0μm)色谱柱;流动相:溶剂A(10mmol/L甲酸铵溶液)和溶剂B(乙腈),梯度洗脱:0-45min,20%B;45-65min,20%-70%B;65-85min,70%-90%B;85-120min,90%B。柱温:30℃;流速:0.8m L/min;质谱采用电喷雾离子源,正离子全扫描检测;通过聚类分析和判别分析进行分类鉴别。结果:建立了不同产地加工方法浙贝母的HPLC-ESI-MS指纹图谱,分别得到38、31、31个共有峰,均推断出了17个化合物;聚类分析和判别分析均能很好地对不同产地加工方法的浙贝母进行分类。结论:所建立的HPLC-ESI-MS指纹图谱,为合理评价和鉴别浙贝母这3种不同产地加工方法提供了可靠手段,为全面控制浙贝母饮片的质量奠定基础。 OBJECTIVE: To establish a HPLC fingerprint chromatogram (HPLC-ESI-MS) fingerprint of Fritillaria cirrhosa from different habitats, and to analyze and identify the constituents. Methods: The mobile phase consisted of solvent A (10 mmol / L ammonium formate solution) and solvent B (acetonitrile) with a gradient of elution from 0 to 45 min, using an Agilent Eclipse plus C18 (4.6 mm × 250 mm, % B; 45-65 min, 20% -70% B; 65-85 min, 70% -90% B; 85-120 min, 90% B. Column temperature: 30 ℃; flow rate: 0.8m L / min; mass spectrometry was performed by electrospray ionization and positive ion scanning; and identified by cluster analysis and discriminant analysis. Results: HPLC-ESI-MS fingerprints of Fritillaria cirrhosae from different habitats were established, and 38, 31 and 31 common peaks were obtained respectively, and 17 compounds were deduced. Both cluster analysis and discriminant analysis Classification of Fritillaria cirrhosa from different processing methods. Conclusion: The established HPLC-ESI-MS fingerprinting provides a reliable means for the rational evaluation and identification of Fritillaria cirrhosae in three different producing areas, which will lay a foundation for the comprehensive control of the quality of Fritillaria speciosa.