厌氧菌依靠发酵取得赖以生存和繁殖的能量。在代谢过程中产生较多的挥发性和非挥发性短链脂肪酸产物,而不同种厌氧菌产生的脂肪酸种类和数量往往不同,因此气相色谱在厌氧菌的鉴定、分类和厌氧菌感染的诊断上具有一定的价值[1,2]. 文献[3,4,5]报道的测定厌氧菌代谢产物中非挥发性或挥发性短链脂肪酸的方法,多先将样品经繁琐、费时的衍生化成它的甲酯(尤其非挥发性酸,如 Anaerobic bacteria rely on fermentation to gain the survival and reproduction of energy. In the metabolic process to produce more volatile and non-volatile short-chain fatty acid products, and different species of anaerobic bacteria often produce different types and amounts of fatty acids, so the identification of anaerobic bacteria, classification and anaerobic infections Of the diagnosis of certain value [1,2]. [3,4,5] reported in the determination of non-volatile or volatile short-chain fatty acids in anaerobic metabolites, the first multi-sample cumbersome, time-consuming Derivatization into its methyl ester (especially non-volatile acids, such as