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目的:探讨整合子介导耐药在多重耐药鲍曼不动杆菌中所起的作用。方法:随机选择120株鲍曼不动杆菌为研究对象,其中超广谱β-内酰胺酶(ESBLs)阳性鲍曼不动杆菌45株为A组,ESBLs阴性鲍曼不动杆菌75株为B组,采用聚合酶链反应(PCR)扩增检测这两组鲍曼不动杆菌的整合酶基因和整合子标志基因,并对这两组扩增产物进行比较分析。结果:A组的int I1整合酶基因和int I2整合酶基因阳性率分别为95.6%(43/45)、88.9%(40/45),类整合子的qac E△1-sul1的阳性率为91.1%(41/45);B组的int I1整合酶基因和int I2整合酶基因阳性率分别为30.7%(23/75),6.7%(5/75),类整合子的qac E△1-sul1的阳性率为22.7%(17/75);A组菌株的整合酶基因和整合子标志基因阳性率显著高于B组菌株,差异均有统计学意义(P<0.05)。结论:鲍曼不动杆菌的整合子耐药基因与ESBLs密切相关,可通过整合子耐药基因及ESBLs检测防止院内感染的区域性流行。
Objective: To investigate the role of integron-mediated drug resistance in multidrug-resistant Acinetobacter baumannii. Methods: 120 strains of Acinetobacter baumannii were selected randomly. Among them, 45 strains of ESBLs-positive Acinetobacter baumannii were group A, 75 strains of ESBLs-negative Acinetobacter baumannii were B The integrase and integron marker genes of Acinetobacter baumannii were detected by polymerase chain reaction (PCR) amplification. The amplification products of the two groups were compared. Results: The positive rates of int I1 integrase gene and int I2 integrase gene in group A were 95.6% (43/45) and 88.9% (40/45), respectively. The positive rate of qac E △ 1-sul1 91.1% (41/45) respectively. The positive rates of int I1 integrase gene and int I2 integrase gene in group B were 30.7% (23/75) and 6.7% (5/75), respectively. The integron qac E △ 1 The positive rate of -sul1 was 22.7% (17/75). The positive rate of integrase gene and integron marker gene in group A was significantly higher than that in group B (P <0.05). CONCLUSION: The integron-resistant genes of Acinetobacter baumannii are closely related to ESBLs, and the regional epidemic of nosocomial infections can be detected by integrating the multidrug resistance genes and ESBLs.