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目的：探讨骨巨细胞瘤（ＧＣＴ）各细胞成分及肿瘤坏死因子α（ＴＮＦα）和白细胞介素１（ＩＬ１）细胞因子在骨吸收中的作用。方法：采用细胞分离技术和体外细胞溶骨模型，检测ＧＣＴ各细胞成分的骨吸收活性及ＴＮＦα和ＩＬ１对这种活性的影响。结果：在溶骨模型中，多核巨细胞（ＭＧＣ）的数目越多，４５Ｃａ２＋的释放也越多；纤维母细胞样基质细胞（ＦＣ）组的４５Ｃａ２＋值明显高于无细胞对照组（Ｐ＜０．０１），而与骨肉瘤细胞组比较，无显著差异；此外，ＦＣ的条件培养液能大大增强ＭＧＣ溶骨模型中４５Ｃａ２＋的释放（Ｐ＜０．０１）；原代单核基质细胞（ＳＣ）经ＴＮＦα处理后，其模型中４５Ｃａ２＋释放明显增高（Ｐ＜０．０１），而ＩＬ１未见类似作用。结论：在ＧＣＴ中，多核巨细胞具有直接溶骨的作用；纤维母细胞样基质细胞，除直接溶骨外，还可以分泌某种物质促进多核巨细胞的溶骨效应；ＴＮＦα能增强原代单核基质细胞的溶骨能力。
Objective: To investigate the role of various cell components of bone cell giant tumor (GCT), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) cytokines in bone resorption. METHODS: Cell separation techniques and in vitro cell lysis model were used to detect the bone resorption activity of various cell components of GCT and the effects of TNF-α and IL-1 on this activity. RESULTS: In the osteolytic model, the greater the number of multinucleated giant cells (MGC), the greater the release of 45Ca2+; the 45Ca2+ value of the fibroblast-like stromal cells (FC) group was significantly higher than that of the non-cell control group (P<0. .01), compared with osteosarcoma cells group, there was no significant difference; In addition, FC conditioned medium can greatly enhance the 45Ca2+ release in the MGC osteolysis model (P<0.01); primary mononuclear stromal cells (SC After treatment with TNFα, 45Ca2+ release was significantly increased in the model (P<0.01), while IL1 had no similar effect. Conclusion: In GCT, multinucleated giant cells have a direct osteolytic effect; fibroblast-like stromal cells, in addition to direct osteolysis, can also secrete certain substances to promote the osteolytic effect of multinuclear giant cells; TNF-α can enhance the original Generation of mononuclear stromal cells with osteolytic potential.