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大肠杆菌胞嘧啶脱氨酶CD可以将前体药物5FC代谢为毒性产物5FU,具有抗肿瘤作用.本课题我们观察了腺病毒介导的CD/5FC自杀基因疗法对小鼠结肠癌生长的治疗作用并探讨其作用机理.结果表明,首先,CD/5FC自杀基因疗法小鼠结肠癌CT26体内外生长均具有显著的抑制作用,4O%的荷瘤小鼠经过治疗后长期存活;其次,我们还研究了自杀基因疗法治疗肿瘤时可能涉及的免疫机理.结果发现在以CD/5FC系统治疗结肠腺癌小鼠过程中能在一定程度上诱导出机体的抗肿瘤免疫反应,包括脾脏CT6L活性的增高、肿瘤局部浸润免疫细胞表达,DEC-205、B7-2、I-A~(b,d)分子增加等.本实验结果提示虽然CD/5FC系统可以诱导机体产生一定的抗肿瘤免疫反应,但其程度不够显著.
E. coli cytosine deaminase CD can metabolize prodrug 5FC into toxic product 5FU, which has anti-tumor effect. We have observed the therapeutic effect of adenovirus-mediated CD/5FC suicide gene therapy on mouse colon cancer growth. The mechanism of action was explored. The results showed that, first of all, CD/5FC suicide gene therapy mice colon cancer CT26 has a significant inhibitory effect in vitro and in vivo, 4O% of tumor-bearing mice survived after long-term survival; secondly, we also studied The immune mechanisms that may be involved in the treatment of tumors by suicide gene therapy have been found to induce anti-tumor immune responses, including increased spleen CT6L activity, to a certain extent during the treatment of colon adenocarcinoma mice with the CD/5FC system. Infiltration of tumor cells with localized expression of immune cells, DEC-205, B7-2, IA-(b, d) molecules increased. The experimental results suggest that although the CD/5FC system can induce the body to produce a certain degree of anti-tumor immune response, but its degree is not enough Significantly.