A better understanding of the effects of a number of environmental factors on denitrification is vital for analyzing its role as nitrogen sink and providing deeper knowledge about the ecological status of a nitrate-rich ecosystem.Since few studies have addressed the occurrence and implications of denitrification in river sediments,and complexity of interactions among all these environmental factors makes comprehension of the process difficult,the potential of sediments from the Deba River to attenuate nitrate excess through denitrification was investigated.For this purpose,we adapted an in vitro method to measure activities of two enzymes contributing to the entire multiple-step nitrate reduction:Nitrate Reductase and Nitrite Reductase.The environmental features that influence both or single enzymatic activities were identified as oxygen availability,regulated directly by the moisture content or indirectly through the aerobic respiration,organic matter and nitrate content of sediments,and electrical conductivity and exchangeable sodium percentage of water.Additionally,our results showed that Nitrate Reductase catalyzes the principal limiting step of denitrification in sediments.Therefore,taking this enzymatic activity as an indicator,the south part of the Deba River catchment presented low potential to denitrify but nitrate-limited sediments,whereas the middle and north parts were characterized by high denitrification potential but nitraterich sediments.In general,this study on denitrifying enzymatic activities in sediments evaluates the suitability of the management of the effluents from wastewater treatment plants and municipal sewages to ensure a good ecological status of the Deba River.