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目的:建立健脾祛湿颗粒(炒白术、干姜、炒苍术、厚朴、陈皮、砂仁等)的质量标准。方法:采用薄层色谱法鉴别方中炒白术、干姜、炒苍术、厚朴、陈皮、砂仁,高效液相色谱法对白术内酯Ⅰ、厚朴酚、和厚朴酚、甘草苷、甘草酸进行定量测定,流动相分别为甲醇-0.1%磷酸水(白术内酯Ⅰ、厚朴酚、和厚朴酚)、乙腈-0.05%磷酸水(甘草苷、甘草酸),检测波长均为237 nm。结果:TLC斑点清晰,分离度好,阴性无干扰。白术内酯Ⅰ、厚朴酚、和厚朴酚、甘草苷和甘草酸分别在0.012 25~0.147 0μg,0.050 50~0.606 0μg,0.073 60~0.883 2μg,0.148 0~1.480μg,0.209 7~2.097μg范围内线性关系良好。结论:本实验定性定量方法简单,结果可靠,所建标准可用于健脾祛湿颗粒的质量控制。
Objective: To establish the quality standard of Jianpi Qushi Granules (Atractylodes macrocephalae, Ginger, Fried herb, Magnolia officinalis, Citrus rehmanniae, Amomum villosum). Methods: The effects of Rhizoma Atractylodis Macrocephalae I, Magnolol, Honokiol, Glycyrrhizin, Rhizoma Atractylodis Macrocephalae, Glycyrrhizic acid was quantitatively determined. The mobile phase consisted of methanol-0.1% phosphoric acid water (atractylenolide I, honokiol, and honokiol respectively) and acetonitrile -0.05% phosphoric acid water 237 nm. Results: TLC spots clear, good resolution, negative non-interference. Atractylodes I, honokiol, honokiol, glycyrrhizin and glycyrrhizin were detected in the range of 0.012 25 ~ 0.147 0μg, 0.050 50 ~ 0.606 0μg, 0.073 60 ~ 0.883 2μg, 0.148 0 ~ 1.480μg, 0.209 7 ~ 2.097μg Linear relationship within the range of good. Conclusion: The qualitative and quantitative methods of this experiment are simple and the results are reliable. The established standards can be used for the quality control of Jianpi Qushi Granules.