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目的 利用绿色荧光蛋白 (greenfluorescentprotein ,GFP)标记的小鼠Lewis肺癌 (LLC)移植瘤模型 ,观察内皮抑素对LLC的作用。方法 将GFP标记的小鼠LLC细胞种植于 15只C5 7小鼠体内 ,分为空白对照组、尾静脉注射内皮抑素组、局部注射内皮抑素组。采用活体荧光成像 ,测定抑瘤率、微血管密度及测定VEGF和Bcl 2水平的方法评价治疗效果。结果 试验组抑瘤率分别为2 1 6 %和 2 7 7% ,较对照组增高 (P <0 0 5 )。尾静脉注射组MVD为 (13 77± 4 4 1)个 /高倍镜视野 ,局部注射组MVD为 (14 13± 4 0 5 )个 /高倍镜视野 ,明显低于空白对照组 (2 7 76± 8 87)个 /高倍镜视野 (P <0 0 1)。微血管计数 (MVD)密度降低 (P <0 0 5 ) ,试验组VEGF和Bcl 2强阳性较对照组少(P <0 0 1)。结论 GFP标记的小鼠LLC模型荧光显像清晰稳定。可在活体观察肿瘤细微的变化过程 ;内皮抑素对小鼠Lewis肺癌移植瘤有明显的抑制作用。
OBJECTIVE: To investigate the effect of endostatin on LLC by using a mouse model of Lewis lung carcinoma (LLC) implanted with green fluorescent protein (GFP). Methods GFP-labeled mouse LLC cells were seeded in 15 C5 7 mice and divided into blank control group, endostatin group and endostatin group. In vivo fluorescent imaging, determination of tumor inhibition rate, microvessel density and determination of VEGF and Bcl 2 levels were evaluated by treatment. Results The tumor inhibition rate of the experimental group was 21.6% and 27.7%, respectively, which was higher than that of the control group (P <0.05). The MVD in the tail vein injection group was (1377 ± 4 4 1) / high magnification and the MVD in the local injection group was (14 13 ± 405) / high magnification, which was significantly lower than that of the control group (27 ± 76) 8 87) / high magnification (P <0.01). Microvessel density (MVD) density was lower (P <0.05), and the positive rates of VEGF and Bcl2 in the experimental group were lower than those in the control group (P <0.01). Conclusion GFP-labeled mouse LLC model fluorescence imaging is clear and stable. In vivo observation of subtle changes in the process; endostatin mouse Lewis lung carcinoma has obvious inhibitory effect.