目的　探索一种简便易行并能同时显示大鼠中枢神经系统 (CNS)神经元和神经纤维的全程连续切片方法。　方法　经过灌流固定的CNS组织低温冰箱速冻后连续冰冻切片和改良的苏木精染色方法。　结果　大鼠全程CNS连续切片各断面上神经元呈蓝黑色 ,神经纤维呈蓝色 ,背景淡黄色。　结论　运用低温速冻、连续冰冻切片、改良的苏木精染色方法可制作同时显示CNS神经元和神经纤维的全程连续切片。 OBJECTIVE: To explore a full-length serial sectioning method for simultaneous display of central nervous system (CNS) neurons and nerve fibers in rats. Methods Frozen CNS tissue cryogenic refrigerator after perfusion fixed frozen sections and improved hematoxylin staining method. Results The neurons in each section of CNS serial sections of rats were blue-black, the nerve fibers were blue and the background was yellowish. Conclusion The whole serial sections of CNS neurons and nerve fibers can be made simultaneously by cryogenic freezing, continuous freezing and modified hematoxylin staining.