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本实验探讨大白鼠肠系膜上动脉夹闭休克时自由基对肠、心、肝、肾、肺等器官组织的细胞损伤作用。实验分对照组(n=6)、夹闭1小时组(n=8)、松夹1小时组(n=8)和松夹2小时组(n=8)。与对照相比,夹闭1小时各器官组织MDA无明显升高(P>0.05);松夹1小时,肠组织MDA增加62%(P<0.05),心肌MDA增加56%(P<0.05);松夹2小时,肠、心、肝、肺等组织MDA分别增加121%(P<0.01)、65%(P<0.05)、32%(P<0.05)和31%(P<0.05)。血浆乳酸、β-葡萄糖醛酸酶和酸性磷酸酶在松夹1小时和2小时均持续显著升高(P<0.01)。结果表明自由基不仅直接导致肠组织的脂质过氧化损伤,同时也可造成肠外生命器官如心、肝、肺等组织的脂质过氧化损伤,从而提示自由基参与肠系膜上动脉夹闭休克时的细胞损伤过程。
This experiment was to investigate the effect of free radicals on the injury of intestine, heart, liver, kidney, lung and other organs and tissues of the superior mesenteric artery of rats. The experimental group was divided into control group (n = 6), clamping for 1 hour (n = 8), clamping for 1 hour (n = 8) and clamping for 2 hours (n = 8). Compared with the control group, MDA in the organs of all organs at 1 hour after clamping was not significantly increased (P> 0.05); the MDA in the intestinal tissue increased by 62% (P <0.05) (P <0.01), 65% (P <0.05), 32% (P <0.05), and 31% (P <0.05) respectively. Plasma lactate, β-glucuronidase and acid phosphatase continued to increase significantly (P <0.01) at 1 hour and 2 hours respectively. The results showed that free radicals not only directly led to lipid peroxidation injury of the intestine, but also caused lipid peroxidation damage in parenteral organs such as heart, liver and lung, suggesting that free radicals may participate in superior mesenteric artery clamping shock When the cell injury process.