目的观察藤梨根提取物对小鼠染色体DNA抗诱变效应,为藤梨根在化疗期间减毒作用提供动物实验依据。方法将昆明种小鼠随机分组,用环磷酰胺(CP)诱变DNA,低、高剂量藤梨根提取物灌胃4d,通过观察小鼠骨髓细胞嗜多染红细胞(PCE)微核(MN)的发生情况,研究藤梨根的抗诱变作用。结果低剂量的藤梨根提取物对环磷酰胺诱发的小鼠骨髓细胞微核发生有明显的抑制效果,MN发生率与模型组比较差异显著,P<0.05。结论藤梨根提取物对环磷酰胺诱变导致的DNA损伤具有保护作用。 Objective To observe the antimutagenic effect of extracts from vine root on mouse chromosomal DNA, and to provide animal experimental evidence for the attenuating effect of vine root during chemotherapy. METHODS: Kunming mice were randomly divided into groups and DNA was mutagenized with cyclophosphamide (CP). Low and high doses of vine root extract were intragastrically administered for 4 days. Mouse bone marrow cells were observed for polychromatic erythrocyte (PCE) micronucleus (MN). To investigate the anti-mutagenicity of vine roots. Results The low-dose vine root extract had obvious inhibitory effect on the micronucleus formation of mouse bone marrow cells induced by cyclophosphamide. The incidence of MN was significantly different from the model group, P<0.05. Conclusion The vine root extract has protective effects on DNA damage induced by cyclophosphamide mutagenesis.