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应用大鼠椎动脉与颈内动脉结扎造成暂时性脑缺血再灌注以及RNA点杂交方法观察c-fos基因与鸟氨酸脱羧酶(ODC)基因表达的动力学过程。结果表明:大鼠大脑皮质c-fos基因在再灌后0.5至3小时表达,ODC基因在6小时至14小时表达;海马的c-fos基因则自36至72小时呈现高水平表达,ODC基因表达与之相对同步或稍延后。但是暂时性脑缺血却不能诱导c-Myc基因表达。上述结果提示c-fos及ODC可能在缺血型脑损伤后具有特殊作用。文中对缺蛋再灌引起cfos-及ODC基因表达的机制进行了分析与讨论,并提出“神经元应激状态”这一概念以描述神经元对伤害性刺激的反应历程。
The kinetic process of c-fos gene and ornithine decarboxylase (ODC) gene expression was observed by transient focal cerebral ischemia-reperfusion and RNA dot blot hybridization in rats with ligation of the vertebral artery and internal carotid artery. The results showed that c-fos gene of rat cerebral cortex was expressed 0.5 to 3 hours after reperfusion, while ODC gene was expressed from 6 hours to 14 hours. The c-fos gene of hippocampus was highly expressed from 36 to 72 hours. ODC gene The expression is relatively synchronous or slightly delayed. However, transient cerebral ischemia failed to induce c-Myc gene expression. These results suggest that c-fos and ODC may have a special role in ischemic brain injury. In this paper, the mechanism of cfos- and ODC gene expression induced by ischemia / reperfusion is analyzed and discussed, and the concept of “neuronal stress state” is proposed to describe the response of neurons to noxious stimuli.