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在牙鲆摄食后1.5、3、5、7和21小时分别检测胃、幽门盲囊和肠道组织中的蛋白酶活性。结果表明,胃蛋白酶的活性在摄食3小时时达到高峰,在摄食后3～5小时,是蛋白质消化由胃向肠道转移的过渡时期,在此期间,胃蛋白酶活性逐渐降低,而肠道蛋白酶活性逐渐增加。摄食后7小时,肠道蛋白酶的活性达到最高值,蛋白质的消化主要由肠道来完成,此时胃蛋白酶活性处于极低水平。幽门盲囊中的蛋白酶活性与胃和肠道密切相关。在下一次摄食前,消化道各部位的蛋白酶活性均处于较高水平。
Protease activities in the stomach, pyloric caecum and intestinal tissue were tested at 1.5, 3, 5, 7, and 21 hours after feeding on flounder. The results showed that the activity of pepsin peaked at 3 hours after ingestion, and during 3 to 5 hours after ingestion, it was the transitional phase from digestion of stomach to intestine. During this period, the activity of pepsin gradually decreased, while the activity of intestinal protease Activity gradually increased. Seven hours after ingestion, the highest level of protease activity in the intestine was achieved. The digestion of the protein was mainly performed by the intestine, at which point the pepsin activity was extremely low. Protease activity in the pyloric caecum is closely related to the stomach and intestine. Before the next feeding, all parts of the digestive tract protease activity are at a high level.